Reporter

Part:BBa_K1468000:Design

Designed by: Pedro Luis Dorado Morales   Group: iGEM14_Valencia_Biocampus   (2014-10-03)


pJ23104 + gene encoding ZsGreen


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 596
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For designing BBa_K1468000 the BBa_J23104 [1] and BBa_E0040 [2] sequences were taken from the registry (see links for more complete information). The biobrick, BBa_K1468004, was contructed by DNA chemical synthesis.

Source

Registry of Standard Biological Parts [3]: BBa_J23104 and BBa_E0040

References

https://parts.igem.org/Part:BBa_E0040

https://parts.igem.org/Part:BBa_J23104

https://parts.igem.org/Main_Page

http://2014.igem.org/Team:Valencia_Biocampus